- The Wind Ensemble encompasses the best student and faculty musicians from the Department of Music and across all majors along with guest conductors to perform wind band literature, transcriptions, and commissioned works.
- The 24-voice College Chorus dates back to 1939 and continues to serve the campus, Upstate community, and audiences all over the world with a large variety of musical selections.
- The Marching Saints began in Spring 2014 with their first marching season in Fall 2014 and is the primary marching ensemble offered at Limestone College. They have performed for football audiences in Upstate SC and Florida, state band competitions, and overseas at the 2014 London New Year's Day parade.
- Saints Percussion is a Percussion Independent Open (PIO) group starting in Spring 2015. Its members consist of percussion/music students at Limestone College and other surrounding high schools and colleges. Saints Percussion is considered an extra-curricular activity, so it is not required by any academic major.
The Vocal Ensemble performs a broad variety of accompanied and acapella music in a variety of settings both on and off campus.
The Brass Ensemble focuses on the development of individual and ensemble skills through performance of both classical and jazz compositions for audiences on and off campus.
The Woodwind Ensemble is dedicated to the study and performance of selected woodwind literature by qualified music majors in different ensemble combinations depending on class enrollment.
The Tuba-Euphonium Ensemble is the primary chamber group for trombone, euphonium and tuba majors, but is open to students from any major who enjoy the jovial camaraderie that is shared by low brass musicians.